Composite
IBR-G22

Part:BBa_K3254005:Design

Designed by: Yeqing Zong, YI REN   Group: iGEM19_GENAS_China   (2019-10-13)


TG1attB-BsaI site-terminator-TG1attP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 48


Design Notes

This part sequence was tested in silico before synthesised to avoid potential promoter activity (see the website in references). A mutation (T to A) was made for avoiding an unexpected BsaI site.


Source

This part was totally synthesised by commerical company.

References

Chen, Y.J., et al., Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods, 2013. 10(7): p. 659-64. http://www.fruitfly.org/seq_tools/promoter.html